Use of Long-Range Repetitive Element Polymorphism-PCR To Differentiate Bacillus anthracis Strains
نویسندگان
چکیده
منابع مشابه
Genetic diversity among Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis strains using repetitive element polymorphism-PCR.
Repetitive element polymorphism-PCR (REP-PCR) is one of the tools that has been used to elucidate genetic diversity of related microorganisms. Using the MB1 primer, REP-PCR fingerprints from 110 Bacillus strains within the "B. cereus group" have identified eighteen distinct categories, while other more distantly related bacterial species fell within six additional categories. All Bacillus anthr...
متن کاملStrategy for identification of Bacillus cereus and Bacillus thuringiensis strains closely related to Bacillus anthracis.
Bacillus cereus strains that are genetically closely related to B. anthracis can display anthrax-like virulence traits (A. R. Hoffmaster et al., Proc. Natl. Acad. Sci. USA 101:8449-8454, 2004). Hence, approaches that rapidly identify these "near neighbors" are of great interest for the study of B. anthracis virulence mechanisms, as well as to prevent the use of such strains for B. anthracis-bas...
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Anthrax is an important zoonotic disease worldwide that is caused by Bacillus anthracis, a spore-forming pathogenic bacterium. A rapid and sensitive method to detect B. anthracis is important for anthrax risk management and control in animal cases to address public health issues. However, it has recently become difficult to identify B. anthracis by using previously reported molecular-based meth...
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There is little published data on the performance of hand-portable polymerase chain reaction (PCR) systems that can be used by first responders to determine if a suspicious powder contains a potential biothreat agent. We evaluated 5 commercially available hand-portable PCR instruments for detection of Bacillus anthracis. We used a cost-effective, statistically based test plan to evaluate system...
متن کاملBacillus anthracis, virulence factors, PCR, and interpretation of results
Editorial Anthrax is a non-contagious infectious disease that affects a wide range of animal species, including humans, but especially sensitive are domestic and wild ruminants. The bacterial agent is Bacillus anthracis, whose main feature is to form spores that can survive in the environment for several decades. Anthrax, in susceptible animals, has a generally fatal outcome characterized by su...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2001
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.67.7.3021-3028.2001